Supreme NZY One-Step RT-PCR Master Mix 2x

Supreme NZY One-Step RT-PCR Master Mix 2x is a robust all-in-one solution designed to deliver exceptional efficiency and sensitivity in cDNA synthesis and PCR amplification in end-point protocols. This ready-to-use formulation enables reverse transcription and PCR to be carried out sequentially in a single tube using gene-specific primers, streamlining experimental workflows and minimizing pipetting errors. At the core of the mix is Supreme NZY Reverse Transcriptase, a second-generation enzyme renowned for its exceptional stability, speed, accuracy, and reproducibility, ensuring high-quality, reliable RT-PCR results. The formulation also includes Supreme NZY Ribonuclease Inhibitor, which safeguards RNA integrity by protecting against RNase-mediated degradation throughout the reaction. For effective cDNA amplification, the mix combines a specially engineered Taq DNA polymerase, optimized for high yield and reaction speed, with a proofreading enzyme that enhances specificity and supports the amplification of long cDNA templates with high accuracy. This unique enzyme blend enables amplification of fragments up to 9 kb, setting a new standard for performance in one-step RT-PCR. The proprietary buffer system supports a universal annealing temperature of 60 °C, significantly reducing the need for optimization across different targets and primer sets. For downstream applications requiring high sensitivity, purification of the amplified product using NZYGelpure (MB011) or Speedy NZY ExoSAP Mix (MB489) is recommended. The amplified products are a mixture of predominantly A-overhang and some blunt-ended fragments, making them fully compatible with NZY-A PCR Cloning kits (MB053 or MB137).