NZY RT SuperMix 5x

NZY RT SuperMix 5x is a ready-to-use next-generation master mix designed for fast and efficient first-strand cDNA synthesis. Its 5x concentrated format simplifies experimental workflows while ensuring high sensitivity and precision, even with low-input RNA or challenging sample types. At the heart of the mix is NZY Reverse Transcriptase (RNase H minus), paired with NZY Ribonuclease Inhibitor, which safeguards RNA integrity by preventing degradation from contaminating RNases throughout the reaction. The mix contains all essential components for robust cDNA synthesis—excluding the RNA template—including dNTPs, an optimized RT buffer, and a dual-priming system. This priming strategy combines random hexamers for broad template coverage and oligo(dT)₁₈ primers for selective poly(A)+ mRNA targeting, offering flexibility across diverse experimental designs. Thanks to its specially formulated enzymes and stabilizers, NZY RT SuperMix 5x enables cDNA synthesis in 10 minutes at 50-55°C, facilitating the melting of RNA secondary structures for efficient transcription. It supports a wide dynamic range of RNA input, from as little as 10 pg to 5 µg of total RNA, ensuring consistent performance across various sample sources and it works Ideal for downstream applications such as real-time PCR, RNA quantification, and gene expression analysis, this advanced mix delivers reproducible, high-quality results with minimal hands-on time, setting a strong standard for easy and consistent cDNA synthesis molecular biology workflows.