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Polaris® Glycerol-free RT-LAMP Reverse Transcriptase 1 kU/μL
Polaris® Glycerol-free RT-LAMP Reverse Transcriptase 1kU/μL is optimized for RT-LAMP and validated across various biological matrices. It presents an increased resistance to inhibitors, reaction kinetics and thermostability. It is lyophilization-compatible. Patent pending submitted by NZYtech.
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Polaris® Glycerol-free RT-LAMP Reverse Transcriptase 1 kU/μL represents a highly optimized, extremely fast reverse transcriptase for reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays. This enzyme is specifically developed to ensure efficient and accurate cDNA synthesis from RNA templates in a RT-LAMP setting, utilizing LAMP primer sets. This enzyme provides optimal conditions for reverse transcription by maintaining high activity and stability under a range of reaction conditions, most notably at around 69 ºC, the optimal temperature range of Polaris® Bst enzymes. Furthermore, it is highly efficient, with cDNA templates being produced in under five minutes in RT-LAMP settings. These features are critical for minimizing the likelihood of errors during RNA transcription, thereby enhancing the yield and specificity of the desired RT-LAMP products, while contributing to a lower probability of non-specific amplification occurrence. It is also extremely resistant to common reaction inhibitors found in typical biological samples. Finally, it is highly compatible with lyophilization procedures, expanding its usability in different contexts. All this results in a highly versatile, efficient and useful enzyme that retains reproducible performance even at lower concentrations. It is highly suited for RT-LAMP applications where rapid transcription and amplification are hallmarks, but without compromising specificity or sensitivity.
Polaris® Glycerol-free RT-LAMP Reverse Transcriptase 1 kU/μL is produced under a rigorous quality management system according to global standards, including stringent quality assurance, full traceability from production, and reliability in its application, meeting a wide range of diagnostic needs in various biological matrices and assay conditions. NZYtech provides the Polaris® Glycerol-free RT-LAMP Reverse Transcriptase 1 kU/μL in a high-concentration, glycerol-free format, allowing for versatility in use, with the help of the accompanying dilution buffer. This allows for ease of utilization whether in discrete assays or to produce other formulations such as Master Mixes. For customers seeking to conduct a smaller scale of experiments, such as 200 reactions, we offer a tailored enzyme pack with everything required to produce a RT-LAMP assay (Polaris® Lyophilizable RT-LAMP Pack, Cat. No. MD07921).
Patent pending submitted by NZYtech.
Polaris® Glycerol-free RT-LAMP Reverse Transcriptase 1 kU/μL is produced under a rigorous quality management system according to global standards, including stringent quality assurance, full traceability from production, and reliability in its application, meeting a wide range of diagnostic needs in various biological matrices and assay conditions. NZYtech provides the Polaris® Glycerol-free RT-LAMP Reverse Transcriptase 1 kU/μL in a high-concentration, glycerol-free format, allowing for versatility in use, with the help of the accompanying dilution buffer. This allows for ease of utilization whether in discrete assays or to produce other formulations such as Master Mixes. For customers seeking to conduct a smaller scale of experiments, such as 200 reactions, we offer a tailored enzyme pack with everything required to produce a RT-LAMP assay (Polaris® Lyophilizable RT-LAMP Pack, Cat. No. MD07921).
Patent pending submitted by NZYtech.
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | RT-LAMP |
Format | Liquid |
Product Category | RT-LAMP |
Compatibility | All standard Real-time thermal cyclers |
Sample Material | RNA |
Protocol time | Approx. 10 min. |
Internal Control | No |
Features | High specificity and stability, Inhibitor resistant, Fast cDNA Synthesis detection (5 min.) |
- Polaris® Glycerol-free RT-LAMP Reverse Transcriptase 1 kU/μL
- DBuffer for Polaris® Glycerol-free RT-LAMP Reverse Transcriptase
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | RT-LAMP |
Format | Liquid |
Product Category | RT-LAMP |
Compatibility | All standard Real-time thermal cyclers |
Sample Material | RNA |
Protocol time | Approx. 10 min. |
Internal Control | No |
Features | High specificity and stability, Inhibitor resistant, Fast cDNA Synthesis detection (5 min.) |
Shipping Conditions | Blue Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | RT-LAMP |
Format | Liquid |
Product Category | RT-LAMP |
Compatibility | All standard Real-time thermal cyclers |
Sample Material | RNA |
Protocol time | Approx. 10 min. |
Internal Control | No |
Features | High specificity and stability, Inhibitor resistant, Fast cDNA Synthesis detection (5 min.) |
CoA
Certificate of Analysis