Cytomegalovirus (Human Herpesvirus 5) qPCR Kit

Cytomegalovirus (CMV) is a genus of viruses that belong to the family of Herpesviridae. Humans and other primates serve as natural hosts for this virus. The 11 species in this genus include Human Herpes Virus 5, which is the species that infects humans. Diseases associated with Human Herpes Virus 5 include mononucleosis and pneumonia.

Cytomegalovirus (Human Herpesvirus 5) qPCR Kit is designed for the in vitro detection of Human CMV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Human CMV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of Human CMV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. However, due to the inherent instability of viral genomes, it is not possible to guarantee detection of all clinical isolates. Other closely related virus are not detected. Real-time PCR detection methods have been used in several studies to identify CMV genomes (Li H. et al 2003).

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.