Clostridium perfringens qPCR Kit

Clostridium perfringens is an anaerobic Gram-positive, rod-shaped, spore-forming pathogenic bacterium of the genus Clostridium. It is associated with acute gastrointestinal infections ranging in severity from diarrhea to necrotizing enterocolitis and myonecrosis in humans. This pathogen possesses an arsenal of toxins responsible for disease pathogenesis and can form spores resistant to environmental stress.

Clostridium perfringens qPCR Kit is designed for the in vitro detection of all five serotypes (A-E) of C. perfringens. The kit is built to have the broadest possible detection profile whilst remaining specific to C. perfringens. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of C. perfringens genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.