Cryptosporidium parvum qPCR Kit

Cryptosporidium parvum is an important gastrointestinal parasite of humans and other animals that can be transmitted via contamination of food and water. This parasite is recognized as a highly infectious enteric pathogen and is the major cause of persistent diarrhea in developing countries.

Cryptosporidium parvum qPCR Kit is designed for the in vitro detection of C. parvum genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to C. parvum. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of C. parvum genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. Real-time PCR detection methods have been used in several studies to identify C. parvum genomes (Higgins J.A. et al 2001).

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.