Entamoeba spp. qPCR Kit

Entamoeba is a genus of single-cell Amoebozoa found as internal parasites or commensals of animals. Most species are found in the intestines of animals, except for Entamoeba gingivalis which commensally lives in the mouth.

Entamoeba spp qPCR Kit is designed for the in vitro detection of Entamoeba spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Entamoeba spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. The primers and probe sequences have very high (>95%) homology with a broad range of Entamoeba spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related genus are not detected. Real-time PCR detection methods have been used in several studies to identify Entamoeba spp. genomes (Santos H.L.C et al 2009).

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.