mScarlet3 mRNA

mScarlet3 mRNA is a ready-to-use messenger RNA (mRNA) product encoding the red fluorescent protein mScarlet3, one of the brightest monomeric red fluorescent proteins currently available. It is characterized by high photostability, rapid folding, and a long fluorescence lifetime. mScarlet3 is suitable for use in mRNA delivery research, fluorescence tracing experiments, protein expression studies, cell transfection assays, live-cell imaging, flow cytometry (fluorescence-activated cell sorting - FACS), and high-throughput screening applications. The transcript is designed for expression in eukaryotic cells. It contains the nucleic acid sequence encoding the 229 amino acid mScarlet3 protein sequence, together with non-coding regulatory elements typically required for efficient translation and stability (5′ untranslated region, 3′ untranslated region, and a 3′ poly(A) tail). The mRNA contains a 5′ Cap 1 structure, and all uridine residues are fully substituted with N1-methyl-pseudouridine (m¹Ψ) to support robust protein expression and to reduce innate immune sensing relative to unmodified mRNA. The mRNA is produced enzymatically by in vitro transcription from a DNA template, followed by capping, polyadenylation, and purification. Promoter sequences used to drive in vitro transcription from the DNA template are not part of the final mRNA product. For research use only (RUO). Not for diagnostic or therapeutic use, and not for human or animal administration.