Mycobacterium spp qPCR Kit

Mycobacterium is a genus of Gram-positive bacteria in the phylum Actinomycetota. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (Mycobacterium tuberculosis) and leprosy (Mycobacterium leprae) in humans.

Mycobacterium spp qPCR Kit is designed for the in vitro detection of Mycobacterium spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Mycobacterium genus. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. The primers and probe sequences have very high (>95%) homology with 85% of Mycobacterium spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. However, the detection of all Mycobacterium strains cannot be guaranteed. Moreover, in silico analysis showed a possible cross reactivity with a number of species belonging to the genera Rhodococcus and Nocardia. Real-time PCR detection methods have been used in several studies to identify Mycobacterium spp. genomes (Radomski N. et al 2010).

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech(see Molecular Diagnostics Homepage. The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.