Mpox Outbreak Declared a Public Health Emergency: Essential Insights for Molecular Diagnostic Labs and Experts

The declaration of Mpox as a Public Health Emergency of International Concern (PHEIC) by the World Health Organization (WHO) has placed laboratory directors, molecular diagnostics experts, and senior scientists at the forefront of the global response. As professionals deeply embedded in the world of molecular biology and diagnostics, your role is critical in ensuring that accurate and reliable testing is available during this escalating crisis.

In response to the urgent need for high-quality diagnostic solutions, we’re offering a 50% discount on our Monkeypox virus Real-time PCR Kit until the end of August. Just add the product to your cart and if your are purchase equals and above 1200€ the 50% discount will automatically apply. For detailed instructions on the kit’s use, please refer to our IFU.

The Evolving Threat of Mpox

Mpox, caused by the Orthopoxvirus, has been a known pathogen since its first identification in humans in 1970 in the Democratic Republic of the Congo (DRC). However, the recent surge in cases, driven by a novel strain (clade 1b), has transformed it from an endemic issue into a potential global health crisis. This strain’s rapid spread through sexual networks in previously unaffected regions, including Burundi, Kenya, Rwanda, and Uganda, presents new challenges for molecular diagnostics.

A Critical Moment for Molecular Diagnostics

The WHO’s declaration highlights the urgent need for precise and scalable diagnostic solutions. With over 15,600 cases and 537 deaths reported to date, the burden falls heavily on laboratory scientists to provide accurate, timely, and reliable diagnostic data. As the virus spreads beyond its traditional endemic regions, laboratories worldwide must be equipped to handle the increased diagnostic demands.

The emergence of clade 1b, which has shown a capacity for rapid transmission, especially through sexual contact, requires an adaptive response from molecular diagnostics teams. Ensuring your lab has access to validated diagnostic kits that meet both regulatory standards and clinical efficacy is critical.

Validated Diagnostic Solutions: The Role of PCR in Mpox Detection

As molecular diagnostic professionals, you understand the critical importance of using validated and reliable testing methods. PCR (Polymerase Chain Reaction) remains the gold standard for detecting Mpox, providing the sensitivity and specificity needed to accurately diagnose and monitor the virus.

Validation by CNR-LE: Ensuring Confidence in Your Diagnostic Tools

In 2023, our Monkeypox PCR Kit was rigorously tested and validated by the Centre national de référence laboratoire expert (CNR-LE) Orthopoxvirus. This validation by one of France’s leading reference laboratories confirms the accuracy and reliability of our kit, ensuring it meets the stringent standards required for diagnostic use.

This validation is a crucial endorsement for labs across Europe and beyond, providing you with the confidence that our kit will deliver reproducible and accurate results in clinical settings. The inclusion of our kit in the CNR-LE’s approved list further underscores its suitability for use in high-stakes diagnostic environments and as an approved diagnostic kit available for sale in France.

For more details on our comprehensive range of diagnostic kits, including those validated by leading authorities, please visit our molecular diagnostics product catalog.

FAQ: Characteristics of a Proper Monkeypox PCR Kit 

1 - What are the key features to look for in a monkeypox PCR kit?

A proper monkeypox PCR kit should have the following characteristics:

• High Specificity: The primers and probes should be highly specific to the monkeypox virus genome, ensuring that only the intended virus is detected. Our kit’s primers and probes have 100% homology with a broad range of monkeypox virus genomes, ensuring precise detection.
• Sensitivity: The kit should be capable of detecting low quantities of viral DNA. Our kit can detect at least 10 copies of the target template per reaction, ensuring high sensitivity.
• Internal Control: The kit should include an internal control to verify sample quality and check for PCR inhibition. Our kit uses an ACTB (β-actin) internal control for this purpose.
• Positive Control: A reliable positive control is essential to validate the assay’s performance. Our kit includes a synthetic positive control to ensure the primers and probes work correctly.
• No Cross-Reactivity: The kit should not cross-react with other closely related viruses. Our kit is designed to specifically target the monkeypox virus, without detecting other Orthopoxvirus species.
• Validated Assay: The kit should be validated to ensure accuracy and reproducibility. Our PCR kit has been meticulously validated for quantitative assays, although the provided positive control is not for quantification.
• Quality Assurance: Look for kits with rigorous quality control measures, including checks for contamination and functional performance. Our kit undergoes strict quality control, including tests for genomic DNA contamination and enzyme activity.

2. Why is it important for the kit to include an internal control?

An internal control, like the ACTB (β-actin) in our kit, is crucial because it:

• Validates Sample Quality: Confirms that nucleic acids are present in the sample and that the PCR reaction is functioning correctly.
• Detects Inhibitors: Identifies potential inhibitors in the sample that could affect PCR performance, ensuring accurate results.

3. How does the kit ensure specificity for the monkeypox virus?

Our PCR kit is designed with primers and probes that are highly specific to the monkeypox virus’s B7R and CrmB genes. This specificity is confirmed through bioinformatics analysis against all reference data in the NCBI database, ensuring that only the monkeypox virus is detected.

4. What are the recommended storage conditions for the kit?

To maintain kit performance:

Shipping & Storage: Store all components at -85°C to -15°C upon arrival.
Avoid Freeze-Thaw Cycles: Minimize freeze-thaw cycles by aliquoting components if necessary.
Protect from Light: Store PPMix protected from light to prevent degradation.

5. Can the kit be used for quantification of the monkeypox virus?

While the kit is designed for quantitative assays, the provided positive control is not intended for quantification. For accurate quantification, we recommend checking our website for the availability of a suitable Quantitative Standard for an accurate quantification

Special Offer: 50% Discount on Monkeypox PCR Kits

To support your efforts during this critical time, we’re offering a 50% discount on orders equal or above 1200€ of the  Monkeypox virus Real-time PCR Kits. This offer is designed to make it easier for your laboratory to access the necessary tools to respond to the Mpox outbreak effectively.

With this discount, you can maintain your budgetary goals while ensuring that your lab is equipped with top-tier diagnostic kits that meet both regulatory requirements and clinical demands. This offer if valid until the end of August.

Comprehensive Support

We understand the importance of technical support in ensuring that your lab staff can confidently utilize our diagnostic kits. That’s why we offer comprehensive and ongoing technical support to assist with the integration of our Monkeypox virus Real-time PCR Kit into your laboratory’s workflow.

Our team of experts, coordinated by an account manager, is available to provide guidance on kit usage, troubleshooting, and best practices, ensuring that your lab can maximize the potential of our diagnostic solutions.

FAQ: The new monkeypox health emergency:

1 - Why is the WHO’s declaration of Mpox as a Public Health Emergency significant for laboratories?  
The declaration emphasizes the urgent need for accurate and scalable diagnostic solutions to manage the outbreak. Laboratories must be equipped with validated kits to ensure timely and reliable diagnosis of Mpox.

2 - How does the validation by CNR-LE impact the use of your Monkeypox virus Real-time PCR Kit? 
The CNR-LE validation confirms that our kit meets the stringent standards required for diagnostic use, ensuring its accuracy and reliability in clinical settings.

3 - What steps should labs take to prepare for increased Mpox testing demands? 
Labs should secure validated diagnostic kits, such as our Monkeypox virus Real-time PCR Kit, and ensure staff are trained in their use. Additionally, scaling up testing capabilities and maintaining regulatory compliance are crucial.

4 - What support is available for labs purchasing your Monkeypox PCR Kits?  
We offer ongoing technical support to ensure that your lab can effectively integrate and use our diagnostic kits.

5 - How long is the 50% discount on Monkeypox PCR Kits available?
The 50% discount is available until the end of August, providing an opportunity to secure high-quality diagnostic kits at a reduced cost during this critical period.

Moving Forward: Ensuring Diagnostic Excellence During the Mpox Crisis

The Mpox outbreak presents significant challenges for molecular diagnostics labs, but with the right tools and support, these challenges can be effectively managed. By choosing validated diagnostic kits like our Monkeypox virus Real-time PCR Kit, and taking advantage of our limited-time discount, you can ensure that your lab is well-equipped to contribute to global health efforts.

This is a crucial moment for laboratory professionals, and your expertise is needed now more than ever. Together, we can navigate the complexities of this outbreak and continue to uphold the highest standards of diagnostic excellence.

Are you searching for different unit sizes or larger volumes to meet your lab's specific needs? We understand that every lab is unique, and we are committed to providing the flexibility and support necessary to ensure you have exactly what you need. Whether you're looking for bulk orders, custom configurations, or special arrangements, we're here to help.

Please click here to submit your request, and our team will work closely with you to tailor our offerings to your exact requirements.

If you have any general questions or need more information about the Monkeypox virus Real-time PCR Kit,, we encourage you to reach out to us through this form. We're here to assist with any inquiries you may have.