Nocardia spp. qPCR Kit

Nocardia is a genus of Gram-positive bacteria that contains a total of 85 species. Some species are nonpathogenic, while others are responsible for nocardiosis. Nocardia species are found in soil rich in organic matter. In addition, they are part of oral microflora found in healthy gingiva, as well as periodontal pockets. Most Nocardia infections are acquired by inhalation of the bacteria or through traumatic introduction. Nocardia spp. can cause opportunistic disease in humans and animals. Nocardiosis is an infectious disease affecting the lungs, but it may spread to other organ systems, most often the brain and the skin.

Nocardia spp. qPCR Kit is designed for the in vitro detection of Nocardia spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Nocardia spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of Nocardia spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. This test may also detect other Actinomycetes.

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase(see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.