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NZY Mag Viral RNA/DNA Isolation Kit, IVD

Storage Conditions:
15 - 25 °C
NZY Mag Viral RNA/DNA Isolation kit, IVD, is a magnetic bead technology‑based nucleic acid purification kit designed to recover RNA and DNA from viral particles contained in transport medium from human nasopharyngeal swabs.
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NZY Mag Viral RNA/DNA Isolation kit, IVD, is a magnetic bead technology‑based nucleic acid purification kit designed to recover RNA and DNA from viral particles contained in transport medium from human nasopharyngeal swabs. The kit uses magnetic-bead technology to provide reproducible recovery of high-quality nucleic acids. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions.

Sample lysis and binding to the NZY Mag Binding Beads is achieved by incubation with NZY Mag Binding Buffer containing chaotropic ions supported by nucleic acid release with NZY Mag Proteinase K digestion. Following nucleic acid magnetic separation, paramagnetic beads holding the RNA/DNA are washed to remove contaminants and salts using NZY Mag Wash Buffer and 80% ethanol. Residual ethanol from previous wash steps is removed by air-drying. Finally, highly pure viral RNA/DNA is eluted with NZY Mag Elution Buffer. It is highly recommended to use extraction controls, positive and negative controls, internal controls as well as to perform cross-contamination assays during the usage of NZY Mag Viral RNA/DNA Isolation kit, IVD.

Protocol options for NZY Mag Viral RNA/DNA Isolation Kit, IVD, support automated workflows in a variety of platforms, including the Thermo ScientificTM KingFisher® Flex Magnetic Particle Processor with a 96 deep-well head. Automated workflows allow for 96 nasopharyngeal swab specimens to be processed in <30 minutes. In addition, a flexible protocol accommodates sample volume inputs of 200 μL of transport medium with an elution volume of 50 μL. High-quality nucleic acids are subsequently ready to use for detection using different amplification strategies, such as Reverse Transcription (RT)-PCR or RT-qPCR and quantitative PCR (qPCR). NZY Mag Viral RNA/DNA Isolation kit, IVD, is intended for use by professional users experienced and trained in molecular biology techniques, including experience with swabs and potentially infectious human sample materials.

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More Information
Shipping Conditions Room Temperature
Storage Conditions 15 - 25 °C
Application cDNA synthesis, In Vitro Diagnostics (IVD), RT-PCR, RT-qPCR
Format Beads
Product Category DNA Purification, RNA Purification
Compatibility TFS KingFisher™ Flex and equivalent
Marking CE-IVD
Isolation Technology Magnetic beads
Protocol time Approx. 30 min
Internal Control No
Features High-throughput (Automated)
More Information
Shipping Conditions Room Temperature
Storage Conditions 15 - 25 °C
Application cDNA synthesis, In Vitro Diagnostics (IVD), RT-PCR, RT-qPCR
Format Beads
Product Category DNA Purification, RNA Purification
Compatibility TFS KingFisher™ Flex and equivalent
Marking CE-IVD
Isolation Technology Magnetic beads
Protocol time Approx. 30 min
Internal Control No
Features High-throughput (Automated)

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Certificate of Analysis

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