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SARS-CoV-2 One-Step RT-qPCR Kit III, 5 Targets, IVD

Storage Conditions:
-85 °C to -15 °C
NZYtech SARS-CoV-2 One-Step RT-qPCR Kit III, 5 Targets, IVD, provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 genome, through common real-time PCR platforms.
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From €576.00
NZYtech SARS-CoV-2 One-Step RT-qPCR Kit III, 5 Targets, IVD, provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 genome, through common real-time PCR platforms. The virus RNA dependent RNA polymerase (RdRp) and the Nucleocapsid phosphoprotein (N) genes have previously been identified as highly specific markers for SARS-CoV-2. In addition, this kit detects a third region located in structural envelope gene E, common to all Sarbecovirus and that is also present in SARS-CoV-2. This NZYtech kit targets specific regions in the RdRp, N and E genes of SARS-CoV-2 genome to provide the highest sensitivity of detection.

One-step RT-qPCR remains the most reliable and sensitive method to perform an accurate detection of SARS-CoV-2 RNA, which is indicative of a human infection. Viral RNA isolated and purified from infected samples is retrotranscribed to cDNA and subsequently amplified in a single reaction using two highly specific primers/probe sets exploiting the so-called TaqMan® principle. During this process, the probes specifically anneal to five regions of the SARS-CoV-2 genome. An additional primers/probe set acts as an endogenous internal control to detect nucleic acids of the human RP gene, assessing sample quality. To allow identifying amplification of the six specific targets in a single reaction, SARS-CoV-2 RdRp, N & E genes and human RP specific probes are differently labelled, with HEX™, FAM™, Texas Red ® and Cy5™ reporter dyes, respectively. Note that this panel contains an hexaplex assay in four distinct optical channels which allow compensating emerging SARS-CoV-2 mutations and variants, ensuring confidence in your results. In addition, primers and probes are provided in optimized concentrations to make sure amplification of human mRNA, even when present at very high concentrations, does not limit the efficiency of SARS-CoV-2 primers/probe sets. In case the IFU is not available in your official country language, please contact us – info@NZYtech.com so that we can proceed with the translation. Please see languages available below.

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More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application In Vitro Diagnostics (IVD)
Format Liquid
Product Category Predesigned qPCR assays
Compatibility AB™ 7500, AB™ QuantStudio 5, Bio-Rad® CFX96™, Roche Life Science LightCycler® 96 System
Marking CE-IVD
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Hexaplex
Internal Control No
Detection Method Probe Based
- NZYSupreme Multiplex One-step RT-qPCR Probe Master Mix (2x)
- SARS-CoV-2 (RdRp, N & E)/RP PPMix III (10x) (HEX™, FAM™, Texas Red® and Cy5™ labelled)
- SARS-CoV-2 (RdRp, N & E)/RP Positive Control 1
- SARS-CoV-2 (RdRp, N & E)/RP Positive Control 2
- No-Template Control
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application In Vitro Diagnostics (IVD)
Format Liquid
Product Category Predesigned qPCR assays
Compatibility AB™ 7500, AB™ QuantStudio 5, Bio-Rad® CFX96™, Roche Life Science LightCycler® 96 System
Marking CE-IVD
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Hexaplex
Internal Control No
Detection Method Probe Based
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application In Vitro Diagnostics (IVD)
Format Liquid
Product Category Predesigned qPCR assays
Compatibility AB™ 7500, AB™ QuantStudio 5, Bio-Rad® CFX96™, Roche Life Science LightCycler® 96 System
Marking CE-IVD
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Hexaplex
Internal Control No
Detection Method Probe Based
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