The NZYEasy Cloning & Expression System was designed to allow directional cloning of PCR-generated fragments or synthetic genes cloned in pUC-vectors into a linearized pHTP vector in a single reaction mediated by the NZYEasy enzyme mix. The system allows achieving high cloning efficiencies (80-100%) and does not require the use of DNA ligases. In addition, no further treatment (e.g. restriction digestion, phosphorylation or blunt-end polishing) of the inserts is required.
NZYTech provides ready-to-use pHTP vectors in separate kits. The NZYEasy Cloning kit (cat. No. MB281) includes the pHTP0 vector (pUC-derivative) for standard cloning reactions, while NZYEasy Cloning & Expression kits include expression vectors that use the T7/lac promoter for regulated high-level protein expression in E. coli strains containing the λDE3 lysogen, such as BL21(DE3). PCR-generated fragments can be cloned into the pHTP0 cloning vector or, alternatively, into one of the various kanamycin-resistant pHTP expression vectors without the need to go through the tedious and laborious intermediate stages. The portfolio of pHTP prokaryotic expression vectors, which includes a different range of fusion tags, offers the possibility to quickly assay levels of expression and solubility of the desired protein in multiple expression vectors simultaneously.
Choose the pHTP vector that most suits your experiment on the table below and see more details on the product specific page: