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β-Acetylglucosaminidase 18A, Bacteroides thetaiotaomicron

Storage Conditions:
-30°C to -15°C
β-Acetylglucosaminidase 18A (BtAcp18A), assigned the E.C. number 3.2.1.96, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure: one N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact. The recombinant BtAcp18A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 18 (GH18) enzyme (see more details at www.cazy.org).
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β-Acetylglucosaminidase 18A (BtAcp18A), assigned the E.C. number 3.2.1.96, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure: one N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact. The recombinant BtAcp18A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 18 (GH18) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Glycoside Hydrolase
Activity Acetylglucosaminidases
Family GH18
EC number 3.2.1.96
Organism Bacteroides thetaiotaomicron
Architecture GH18
Optimal pH 7.5
Optimal Temperature 37°C
Substrates mammalian high mannose N-glycans (HMNG), Man9GlcNAc2
Cuskin et al. (2015) Nature 517, 165-169.
Mahowald et al. (2009) Proc Natl Acad Sci U S A. 106(14):5859-5864.
Trastoy et al. (2020) Nat Commun. 11(1):899.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Glycoside Hydrolase
Activity Acetylglucosaminidases
Family GH18
EC number 3.2.1.96
Organism Bacteroides thetaiotaomicron
Architecture GH18
Optimal pH 7.5
Optimal Temperature 37°C
Substrates mammalian high mannose N-glycans (HMNG), Man9GlcNAc2
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Glycoside Hydrolase
Activity Acetylglucosaminidases
Family GH18
EC number 3.2.1.96
Organism Bacteroides thetaiotaomicron
Architecture GH18
Optimal pH 7.5
Optimal Temperature 37°C
Substrates mammalian high mannose N-glycans (HMNG), Man9GlcNAc2

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