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β-Acetylglucosaminidase 18A, Bacteroides thetaiotaomicron
β-Acetylglucosaminidase 18A (BtAcp18A), assigned the E.C. number 3.2.1.96, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure: one N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact. The recombinant BtAcp18A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 18 (GH18) enzyme (see more details at www.cazy.org).
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β-Acetylglucosaminidase 18A (BtAcp18A), assigned the E.C. number 3.2.1.96, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure: one N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact. The recombinant BtAcp18A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 18 (GH18) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
Shipping Conditions | Shipping: Dry Ice to RT |
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Storage Conditions | -30°C to -15°C |
Class | Glycoside Hydrolase |
Activity | Acetylglucosaminidases |
Family | GH18 |
EC number | 3.2.1.96 |
Organism | Bacteroides thetaiotaomicron |
Architecture | GH18 |
Optimal pH | 7.5 |
Optimal Temperature | 37°C |
Substrates | mammalian high mannose N-glycans (HMNG), Man9GlcNAc2 |
Cuskin et al. (2015) Nature 517, 165-169.
Mahowald et al. (2009) Proc Natl Acad Sci U S A. 106(14):5859-5864.
Trastoy et al. (2020) Nat Commun. 11(1):899.
Mahowald et al. (2009) Proc Natl Acad Sci U S A. 106(14):5859-5864.
Trastoy et al. (2020) Nat Commun. 11(1):899.
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Glycoside Hydrolase |
Activity | Acetylglucosaminidases |
Family | GH18 |
EC number | 3.2.1.96 |
Organism | Bacteroides thetaiotaomicron |
Architecture | GH18 |
Optimal pH | 7.5 |
Optimal Temperature | 37°C |
Substrates | mammalian high mannose N-glycans (HMNG), Man9GlcNAc2 |
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Glycoside Hydrolase |
Activity | Acetylglucosaminidases |
Family | GH18 |
EC number | 3.2.1.96 |
Organism | Bacteroides thetaiotaomicron |
Architecture | GH18 |
Optimal pH | 7.5 |
Optimal Temperature | 37°C |
Substrates | mammalian high mannose N-glycans (HMNG), Man9GlcNAc2 |
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Certificate of Analysis