Description: SpAcp85A (GH85), E.C. number 220.127.116.11, is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)(2))Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact from Streptococcus pneumoniae. Recombinant SpAcp85A (GH85), purified from Escherichia coli, is a single domain family 85 Glycoside Hydrolase (GH85) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: – 20 °C
Temp: 37 °C
Substrates: chitobiose core of N-glycans and the artificial substrates 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (3F4NP-GlcNAc)