Description: CmMns5A (GH5), E.C. number 22.214.171.124, is an exo-1,4-β-mannosidase that removes mannose residues from the non-reducing ends of oligosaccharides from Cellvibrio mixtus. Recombinant CmMns5A (GH5), purified from Escherichia coli, is a single domain family 5 Glycoside Hydrolase (GH5) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 3 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: -20 °C
pH: 6.5 – 7.5
Temp: 37 °C
Substrates: mannose from the non-reducing end of mannooligosaccharides and polysaccharides