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β-Mannosidase 1A, Pyrococcus furiosus
β-Mannosidase 1A (PfMns1A), assigned the E.C. number 3.2.1.25, is a derivative of Pyrococcus furiosus. It is an exo-1,4-β-mannosidase that removes mannose residues from the non-reducing ends of oligosaccharides. The recombinant PfMns1A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 1 (GH1) enzyme (see more details at www.cazy.org).
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Produced On Demand
β-Mannosidase 1A (PfMns1A), assigned the E.C. number 3.2.1.25, is a derivative of Pyrococcus furiosus. It is an exo-1,4-β-mannosidase that removes mannose residues from the non-reducing ends of oligosaccharides. The recombinant PfMns1A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 1 (GH1) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
Shipping Conditions | Shipping: Dry Ice to RT |
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Storage Conditions | -30°C to -15°C |
Class | Glycoside Hydrolase |
Activity | β-Mannosidases |
Family | GH1 |
EC number | 3.2.1.25 |
Organism | Pyrococcus furiosus |
Architecture | GH1 |
Optimal pH | 7.4 |
Optimal Temperature | 105°C |
Substrates | 4-nitrophenyl-α-glucopyranoside (GlcpbNp), 4-nitrophenyl-β-D-mannopyranoside (ManpbNp) |
Bauer et al. (1996) J Biol Chem. 271(39):23749-55.
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Glycoside Hydrolase |
Activity | β-Mannosidases |
Family | GH1 |
EC number | 3.2.1.25 |
Organism | Pyrococcus furiosus |
Architecture | GH1 |
Optimal pH | 7.4 |
Optimal Temperature | 105°C |
Substrates | 4-nitrophenyl-α-glucopyranoside (GlcpbNp), 4-nitrophenyl-β-D-mannopyranoside (ManpbNp) |
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Glycoside Hydrolase |
Activity | β-Mannosidases |
Family | GH1 |
EC number | 3.2.1.25 |
Organism | Pyrococcus furiosus |
Architecture | GH1 |
Optimal pH | 7.4 |
Optimal Temperature | 105°C |
Substrates | 4-nitrophenyl-α-glucopyranoside (GlcpbNp), 4-nitrophenyl-β-D-mannopyranoside (ManpbNp) |
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Certificate of Analysis