Description: NIft91A (GH91), E.C. number 4.2.2.18, is an enzyme that produces α-D-fructofuranose β-D-fructofuranose 1,2′:2,3′-dianhydride (DFA III) by successively eliminating the diminishing (2->1)-β-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide from Nonomuraea sp.. Recombinant NIft91A (GH91), purified from Escherichia coli, is a single domain family 91 Glycoside Hydrolase (GH91) (www.cazy.org). The protein is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2 and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: -20 °C