Rhamnogalacturonan rhamnohydrolase 106A (CZ0848)

Activity: Family: Organism: Bacteroides thetaiotaomicron EC Number: 3.2.1.174 Architecture: GH106

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Description: BtRhh106A (GH106), E.C. number 3.2.1.174, is an enzyme that participates in the exohydrolysis of the α-L-Rha-1,4-α-D-GalA bond in rhamnogalacturonan oligosaccharides with initial inversion of configuration releasing β-L-rhamnose from the non-reducing end of rhamnogalacturonan oligosaccharides from Bacteroides thetaiotaomicron. Recombinant BtRhh106A (GH106), purified from Escherichia coli, is a single domain family 106 Glycoside Hydrolase (GH106) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.

Storage: – 20 °C

Architecture: GH106

pH: 6,5-7,5

Temp: 37 °C

Substrates: the L-Rha-α4-D-GalA linkage in rhamnogalacturonan I (RGI)

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