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α-Rhamnosidase 78B & Kdo hydrolase 33A, Bacteroides thetaiotaomicron

Storage Conditions:
-30°C to -15°C
α-Rhamnosidase 78B & Kdo hydrolase 33A (BtRam78B-Kdo33A), assigned the E.C. number 3.2.1.40 and 3.2.1.- , is a derivative of Bacteroides thetaiotaomicron. It is a bifunctional enzyme that participates in the hydrolysis of terminal non-reducing α-L-rhamnose residues in α-L-rhamnosides and a 2-keto-3-deoxynononic acid hydrolase. The recombinant BtRam78B-Kdo33A, purified from Escherichia coli, is a modular Glycoside Hydrolase family 78 and 33 (GH78 and GH33) enzyme, containing GH78 and GH33 catalytic domains and an N-terminal family 67 Carbohydrate Binding Module (CBM67) (see more details at www.cazy.org).
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Produced On Demand
From €199.00
α-Rhamnosidase 78B & Kdo hydrolase 33A (BtRam78B-Kdo33A), assigned the E.C. number 3.2.1.40 and 3.2.1.- , is a derivative of Bacteroides thetaiotaomicron. It is a bifunctional enzyme that participates in the hydrolysis of terminal non-reducing α-L-rhamnose residues in α-L-rhamnosides and a 2-keto-3-deoxynononic acid hydrolase. The recombinant BtRam78B-Kdo33A, purified from Escherichia coli, is a modular Glycoside Hydrolase family 78 and 33 (GH78 and GH33) enzyme, containing GH78 and GH33 catalytic domains and an N-terminal family 67 Carbohydrate Binding Module (CBM67) (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Glycoside Hydrolase
Activity Rhamnogalacturonases & Other Activities
Family GH33, GH78
EC number 3.2.1.40 and 3.2.1.-
Organism Bacteroides thetaiotaomicron
Architecture CBM67-GH78-GH33
Optimal pH 7
Optimal Temperature 37°C
Substrates 4-nitrophenyl-α-L-rhamnose, rhamnogalacturonan II
Ndeh et al. (2017) Nature. 544(7648):65-70.
Mahowald et al. (2009) Proc Natl Acad Sci U S A. 106(14):5859-64.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Glycoside Hydrolase
Activity Rhamnogalacturonases & Other Activities
Family GH33, GH78
EC number 3.2.1.40 and 3.2.1.-
Organism Bacteroides thetaiotaomicron
Architecture CBM67-GH78-GH33
Optimal pH 7
Optimal Temperature 37°C
Substrates 4-nitrophenyl-α-L-rhamnose, rhamnogalacturonan II
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Glycoside Hydrolase
Activity Rhamnogalacturonases & Other Activities
Family GH33, GH78
EC number 3.2.1.40 and 3.2.1.-
Organism Bacteroides thetaiotaomicron
Architecture CBM67-GH78-GH33
Optimal pH 7
Optimal Temperature 37°C
Substrates 4-nitrophenyl-α-L-rhamnose, rhamnogalacturonan II

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