Description: CpNig65A (GH65), E.C. number 188.8.131.529, is a nigerose phosphorylase from Clostridium phytofermentans. Recombinant CpNig65A (GH65), purified from Escherichia coli, is a single domain family 65 Glycoside Hydrolase (GH65) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 0,25 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: – 20 °C
Temp: 40 °C
Substrates: nigerose and shows weak phosphorolytic activity on kojibiose. The enzyme also showed reverse phosphorolytic activity, in a decreasing order, on D-xylose, 1,5-anhydro-D-glucitol, D-galactose, and methyl-α-D-glucoside.