Nigerose phosphorylase 65A (CZ0956)

Activity: Family: Organism: Clostridium phytofermentans EC Number: Architecture: GH65



Description: CpNig65A (GH65), E.C. number, is a nigerose phosphorylase from Clostridium phytofermentans. Recombinant CpNig65A (GH65), purified from Escherichia coli, is a single domain family 65 Glycoside Hydrolase (GH65) ( The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 0,25 mg/mL concentration. Bulk quantities of this product are available on request.

Storage: – 20 °C

Architecture: GH65

pH: 7

Temp: 40 °C

Substrates: nigerose and shows weak phosphorolytic activity on kojibiose. The enzyme also showed reverse phosphorolytic activity, in a decreasing order, on D-xylose, 1,5-anhydro-D-glucitol, D-galactose, and methyl-α-D-glucoside.

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