Description: BtHep12A (PL12), E.C. number 220.127.116.11, is an enzyme from Bacteroides thetaiotaomicron that acts on linkages between acetyl-glucosamine and uronate. Recombinant BtHep12A (PL12), purified from Escherichia coli, is a single domain family 12 Pectate Lyase (PL12) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: -20 °C
Temp: 37 °C
Substrates: heparan sulfate (HS) polysaccharides