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Heparin-sulfate lyase 12A, Pedobacter heparinus

Storage Conditions:
-30°C to -15°C
Heparin-sulfate lyase 12A (PhHep12A), assigned the E.C. number 4.2.2.8, is a derivative of Pedobacter heparinus. It is an enzyme that participates in the eliminative cleavage of Heparan sulfate by putativaly acting on the linkages between acetyl-glucosamine and uronate. The recombinant PhHep12A, purified from Escherichia coli, is a single-domain Pectate Lyase family 12 (PL12) enzyme (see more details at www.cazy.org).
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From €199.00
Heparin-sulfate lyase 12A (PhHep12A), assigned the E.C. number 4.2.2.8, is a derivative of Pedobacter heparinus. It is an enzyme that participates in the eliminative cleavage of Heparan sulfate by putativaly acting on the linkages between acetyl-glucosamine and uronate. The recombinant PhHep12A, purified from Escherichia coli, is a single-domain Pectate Lyase family 12 (PL12) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Polysaccharide Lyase
Activity Heparin lyases
Family PL12
EC number 4.2.2.8
Organism Pedobacter heparinus
Architecture PL12
Optimal pH 7.5
Optimal Temperature 35°C
Substrates heparan sulfate
Godavarti et al. (1996) Biochem Biophys Res Commun. 225(3):751-8.
Su et al. (1996) Appl Environ Microbiol. 62(8):2723-34.
Hashimoto et al. (2014) Biochemistry. 53 (4), pp 777–786.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Polysaccharide Lyase
Activity Heparin lyases
Family PL12
EC number 4.2.2.8
Organism Pedobacter heparinus
Architecture PL12
Optimal pH 7.5
Optimal Temperature 35°C
Substrates heparan sulfate
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Polysaccharide Lyase
Activity Heparin lyases
Family PL12
EC number 4.2.2.8
Organism Pedobacter heparinus
Architecture PL12
Optimal pH 7.5
Optimal Temperature 35°C
Substrates heparan sulfate

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