Rhamnogalacturonan endolyase 9B (CZ0186)

Activity: Family: Organism: Bacteroides thetaiotaomicron EC Number: Architecture: PL9



Description: BtRge9B (PL9), E.C. number, is an an enzyme that participates in the endotype eliminative cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end from Bacteroides thetaiotaomicron. Recombinant BtRge9B (PL9), purified from Escherichia coli, is a single domain family 9 Pectate Lyase (PL9) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.

Storage: -20 °C

Architecture: PL9

pH: 9

Temp: 37 °C

Substrates: rhamnogalacturonan I

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