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Rhamnogalacturonan endolyase 9A, Bacteroides thetaiotaomicron

Storage Conditions:
-30°C to -15°C
Rhamnogalacturonan endolyase 9A (BtRge9A), assigned the E.C. number 4.2.2.23, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endotype eliminative cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant BtRge9A, purified from Escherichia coli, is a single-domain Pectate Lyase family 9 (PL9) enzyme (see more details at www.cazy.org).
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Rhamnogalacturonan endolyase 9A (BtRge9A), assigned the E.C. number 4.2.2.23, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endotype eliminative cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant BtRge9A, purified from Escherichia coli, is a single-domain Pectate Lyase family 9 (PL9) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Polysaccharide Lyase
Activity Rhamnogalacturonan lyases
Family PL9
EC number 4.2.2.23
Organism Bacteroides thetaiotaomicron
Architecture PL9
Optimal pH 7.5
Optimal Temperature 37°C
Substrates rhamnogalacturonan I
Luis et al. (2018) Nat Microbiol. 3(2):210-219.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Polysaccharide Lyase
Activity Rhamnogalacturonan lyases
Family PL9
EC number 4.2.2.23
Organism Bacteroides thetaiotaomicron
Architecture PL9
Optimal pH 7.5
Optimal Temperature 37°C
Substrates rhamnogalacturonan I
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Polysaccharide Lyase
Activity Rhamnogalacturonan lyases
Family PL9
EC number 4.2.2.23
Organism Bacteroides thetaiotaomicron
Architecture PL9
Optimal pH 7.5
Optimal Temperature 37°C
Substrates rhamnogalacturonan I

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