Description: Supreme NZYLong DNA polymerase is an engineered version of NZYLong DNA polymerase designed to amplify longer PCR products, generally of 25 kb and beyond, which displays a higher specificity provided by its hot-start-like PCR capacity. This feature is achieved by a novel hot-start technology, which inhibits both polymerase and 3’→5’ exonuclease activities and thus avoids extension of non-specifically annealed primers or primer-dimers, as well as degradation of primers and template DNA during PCR reaction setup. The functional activity of the enzyme is restored during a short 5-minute incubation step at 94 °C. The increased processivity of Supreme NZYLong DNA polymerase combined with the hot-start-like PCR capacity results in higher specificity, sensitivity and yield during the amplification of long nucleic acids. A wide range of long PCR products, typically of 25 kb and up to 40 kb, can be generated usimg lambda DNA or human genomic DNA as starting template. Supreme NZYLong DNA polymerase displays a higher fidelity than conventional Taq DNA polymerases. The provided 10x reaction buffer protects DNA from depurination and nicking during long thermal cycling. The enzyme generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products, being suitable for cloning with NZYTech´s TA PCR cloning kits (MB053 or MB137).
– Amplifies long DNA fragments
– High specificity: Eliminates nonspecific amplifications and minimizes primer-dimers
– Higher fidelity than NZYTaq DNA polymerase
– Long-Range PCR
– Generation of products for TA cloning
Superior sensitivity with large-sized fragments
Supreme NZYLong DNA polymerase exhibits a great performance in the presence of low amounts of starting template, even to amplify large-sized DNA fragments.
A 10 kb DNA fragment was amplified using 2-fold Escherichia coli genomic DNA dilutions ranging from 40 ng to 39 pg (lanes 1-11) with Supreme NZYLong DNA polymerase. NC: No template control. Lane M: NZYDNA Ladder III.
|Product length:||0-20 kb|
|Hot-start like capacity:||yes|
|Extension time:||60 sec/kb at 68 °C|
|Available as Mixes:||Supreme NZYLong 2x Green Master Mix (MB333); Supreme NZYLong 2x Colourless Master Mix (MB334)|
|Storage conditions:||Store at -20 ºC|
|Shipping conditions:||Shipped at 4 ºC to dry ice|
– Supreme NZYLong DNA polymerase (5 U/μL)
– Reaction buffer (10x)