Description: NZYTaq II DNA polymerase belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. The enzyme was engineered to produce high DNA yields in shorter PCR running times (15-30 s/kb extension) under minimal optimization conditions. NZYTaq II DNA polymerase lacks 3´→5´ exonuclease activity and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. The enzyme was optimized to provide higher sensitivity, allowing amplification of different DNA fragments from as little as 5 pg of human genomic DNA. Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).
– High yields with minimal optimization
– Robust amplification using a wide range of DNA templates
– Leaves an A-overhang
– Powerful PCR
– Generation of products for TA cloning
|Product length:||0-6 kb|
|Extension time:||15-30 sec/kb at 72 ºC|
|Available as Mixes:||NZYTaq II 2x Green Master Mix (MB358); NZYTaq II 2x Colourless Master Mix (MB357)|
|Storage conditions:||Store at -20 ºC|
|Shipping conditions:||Shipped at 4 ºC to dry ice|
– NZYTaq II DNA polymerase (5 U/μL)
– Reaction buffer (10x)
– MgCl2 Solution (50 mM)
NZYTech also provides a hot-start-like version of NZYTaq II DNA polymerase (Supreme NZYTaq II DNA polymerase), for powerful amplification of low-copy templates.