Description: Speedy Supreme NZYTaq DNA polymerase is a recombinant modified form of Taq DNA polymerase that combines hot-start like PCR capacity and a fast polymerization reaction. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity and sensitivity of DNA amplification. The functional activity of the enzyme is restored during a short 5- minute incubation step at 95 °C. Speedy Supreme NZYTaq DNA polymerase is supplied with an optimized 10× Reaction Buffer and a 50 mM MgCl2 solution. Minimal optimizations are required for successful DNA amplification. In addition, the hot-start-like capacity of Speedy Supreme NZYTaq DNA polymerase not only leads to higher PCR sensitivity but also allows a room-temperature reaction setup. The enzyme lacks 3´→5´ exonuclease activity. Thus, resulting PCR products have an A-overhang and are suitable for cloning with NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).
– Reproducible and convenient (provides the convenience of room temperature reaction set-up)
– Fast routine amplification (5 sec/kb for DNA fragments up to 2-3 kb)
– Eliminates nonspecific amplifications and minimizes primer-dimers
– Robust amplification using a wide range of DNA templates
– Leaves an A-overhang
– Fast PCR
– Powerful PCR
– Amplification of low-copy templates
– High specificity amplifications
– Hot-start PCR
– Generation of products for TA cloning
High sensitive and specific amplification of diferent-sized DNA fragments using a fast PCR protocol
The elongation rate, as well as the specificity, of Speedy Supreme NZYTaq DNA polymerase was tested in a fast PCR assay to amplify fragments of 1 kb, 2.5 kb and 5kb from different dilutions of human genomic DNA using two amounts of enzyme (1 and 0.5 µL). The protocol provided with the Speedy Supreme NZYTaq DNA polymerase was followed using a limited extension period of 5 sec/kb to amplify the 1 kb fragment and 10 sec/kb to amplify both 2.5 kb and 5 kb.
A 5-fold human genomic DNA dilution, ranging from 20 to 0.032 ng, was used for the amplification of different-sized fragments. Lane M: NZYDNA Ladder III. PCRs were performed in 50 µL reaction mixtures with elongation times of 5 sec, 25 sec, and 50 sec for 1kb, 2.5 kb and 5 kb fragments, respectively. Results show successful amplification in conditions under test.
|Product length:||0-6 kb|
|Fidelity (vs. Taq):||1×|
|Extension time:||5 sec/kb (or 10 sec/kb for targets higher than 2-3 kb in size) at 72 °C|
|Available as Mixes:||Speedy Supreme NZYTaq 2x Green Master Mix (MB91); Speedy Supreme NZYTaq 2x Colourless Master Mix (MB392)|
|Storage conditions:||Store at -20 ºC|
|Shipping conditions:||Shipped at 4 ºC to dry ice|
– Speedy Supreme NZYTaq DNA polymerase (5 U/μL)
– Reaction buffer (10x)
– MgCl2 Solution (50 mM)