BlueSafe

BlueSafe (MB15201)

Quantity: 1000 mL
€99,00

Description: : BlueSafe is a protein stain which consists of a safer alternative to the traditional Coomassie Blue staining for detecting proteins in SDS-PAGE. BlueSafe is a highly sensitive single step protein stain. It is safer than Coomassie Blue staining because does not contain any methanol or acetic acid in its composition. Furthermore, it does not require the use of a destaining solution. Simply run a SDS-PAGE, add the stain and watch the bands appear in a few minutes. There is no need to wash or destain and the background is crystal clear. BlueSafe will show low concentration proteins (60 ng) within 10-15 minutes and down to 10 ng within 30-60 minutes. The signal generated by this stain is linear, enabling true semi-quantification of the sample.

Features:
– Ready-to-use
– Nonhazardous
– High sensitivity
– Produces blue bands on a crystal clear background
– Simple and quick staining protocol
– Reduces reagent waste disposals

Applications:
– Visualization of protein patterns in polyacrylamide gels after electrophoresis

High contrast and sensitivity of BlueSafe:
BlueSafe

Specifications:

Target molecule: Proteins
Mode of use: Post-staining
Disposal: Waste must be disposed in accordance with environmental control regulations
Storage conditions: Store at 4°C, protected from light
Shipping conditions: Shipped at room temperature to 4 ºC
MSDS EN
MSDS PT
Product Brochure EN

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Featured citations
The whole proteome Coomassie Brilliant Bluestain, BlueSafe (CBB) and the protein molecular weight markers NZYColour Protein Marker II were acquired from NZYTech (Portugal).
Luís, I.M., Alexandre, B.M., Oliveira, M.M., Abreu, I.A. (2016). Selection of an Appropriate Protein Extraction Method to Study the Phosphoproteome of Maize Photosynthetic Tissue. PLoS ONE 11(10): e0164387

1. What does BlueSafe bind to?                  
BlueSafe binds to basic amino acids in a similar way traditional Coomassie Blue does, but in a faster and more sensitive way.

2. Why is it important better to incubate the protein gels with BlueSafe at room temperature?                      
BlueSafe works much more quickly at warmer temperatures. Actually, it can take 10x longer to visualize bands when it is cold.

3. What is the optimal temperature for staining gels?                  
For achieving the most effective staining, we recommend preheating BlueSafe (~25ml) to a temperature of 37°C.

4. Can I decrease the time for staining?                  
Yes. By inserting gel with BlueSafe into microwave for 15 seconds.

5. After staining, how can I store the gel?              
You can store the gel in distilled water or in alternative in acetic acid.

6. Can proteins stained with BlueSafe be used in mass spec analysis?            
Yes. Please see BlueSafe product brochure for more details.

7. Is BlueSafe compatible with a western blot?                 
Yes. You will need to wash the gel a few times in transfer buffer. You can also stain the gel after blotting as this is a good method of determining transfer efficiency.

8. Can BlueSafe be re-used?             
Yes. It can typically be re-used two or three times.

 9. Can BlueSafe also be used to dye protein native gels?
Yes. BlueSafe is also suitable for protein native gels.

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