EDTA 0.5 M solution, pH 8.0 (liquid) (MB25102)

88,00

250 mL

88,00

Description: EDTA (ethylene-diamine-tetraacetic acid) is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium. This ability prevents DNA and RNA degradation as metal-dependent enzymes acting as nucleases becomes deactivated. A fully deprotonated EDTA molecule will bind directly to the metal ion making the buffer suitable for adding to stored blood as an anti-coagulant to bind Ca2+ ions. Furthermore, EDTA is useful for cell culture procedures as it prevents clumping of cells in liquid suspension and detaches adherent cells when passaging.

Applications:
– Added to stored blood as an anticoagulant
– Inhibits metal-dependent enymatic reactions
– Prevents cell-to-cell joining of cadherins
– Used in electrophoresis buffers TAE and TBE
– Component in TE buffers for DNA and RNA applications
– Cell culture procedures

Specifications:
– Soluble in water

Storage: Store at 2-8 °C

MSDS EN
MSDS PT