TE Buffer 10x, pH 7.4 (powder) (MB11301)

Description: Tris-EDTA (TE) buffer is composed by Tris, a buffering agent and EDTA, a chelating agent. EDTA has the ability to prevent degradation of DNA and RNA by chelating magnesium- or other divalent metal ions.Tris-EDTA-based solutions break protein cross-links and can therefore unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. Treatment with TE buffer enhances the staining intensity of antibodies in the immuno-histochemical detection of certain proteins.

NZYTech’s TE buffer is supplied as exactly pre-weighed powder in pouches, giving 1000 mL of 0.1 M Tris-HCl, 0.010 M Tris-EDTA buffer with pH 7.4 at 25 °C when dissolved in nuclease free water or deionized water.

Applications:
– Storing and diluting DNA and RNA
– Breaking protein-links in immuno-histochemistry procedures

Directions for use: Empty one pouch of the TE buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 mL f deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 mL and the buffer solution is ready to use.

Specifications:
– Chemical: Analytical grade
– Format: Exactly pre-weighed powder mix
– Concentration: 0.1 M Tris-HCl, 0.010 M EDTA
– Volume: for 1000 mL
– pH: 7.4 ± 0.05 at 25 °C

Storage: Store the pouches in a dry place at room temperature.