Description: In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TBE (Tris-Borate-EDTA) buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels.
NZYTech’s TBE buffer is supplied as a pre-weighed powder mix in sealed pouches giving 1000 mL 10x Tris-borate-EDTA buffer with pH 8.3 at 25 °C.
Applications:
– Nucleic acid electrophoresis running buffer for agarose and polyacrylamide gels
Directions for use: Empty one pouch of the TBE buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 mL of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 mL, stir until full dissolution and the buffer solution is ready to use.
Specifications:
– Chemicals: Analytical grade
– RNase/DNase activity: Non-detectable
Storage: Store the pouches in a dry place at room temperature.
