Description: Tris Buffered Saline (TBS) is isotonic and non-toxic to cells and is suitable for molecular biology. The buffer is commonly used as substance diluent or as wash buffer in immunoassays such as ELISA. It is used in immuno-histochemical staining when the background is high and for diluting alkaline phosphatase or peroxidase-conjugated antibodies in Western blotting.
NZYTech’s TBS buffer is supplied as powder in sealed pouches. The contents of one pouch gives 1000 ml of buffer solution in the composition 0.5 M Tris buffered saline, 1.38 M sodium chloride, 0.027 M potassium chloride with pH 8.0 at 25 °C.
Features:
–Â Formulated from analytical grade reagents
– Isotonic, non-toxic buffer
– Guaranteed reproducibility
– Ready to use in minutes
Applications:
–Â Wash buffer in immunoassays
– Antibody diluent in Western blotting
– Sample diluent in in vitro diagnostics
– Immuno-histochemical staining
– In Situ hybridization
Specifications:
– Chemicals: Analytical grade
– Format: Exactly pre-weighed powder mix
– Concentration: 0.5 M Tris buffered saline, 1.38 M sodium chloride, 0.027 M potassium chloride
– Volume: 1000 mL
– pH: 8.0 at 25 °C
Directions for use: Empty one pouch of the buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 500 mL deionized
water and stir the solution for a few minutes. Adjust the volume up to 1000 mL, stir until full dissolution and the buffer solution is ready to use.
Storage: Store the pouches in a dry place at room temperature.
