Description: Tris buffer is used in many applications within biochemistry and molecular biology laboratories and is a component in TAE and TBE (electrophoresis running buffer). Tris has a slightly alkaline buffering capacity between pH 7.0 and 9.2, which coincides with the typical physiological pH of most living organisms. Tris buffer is a good option for washing procedures in cell cultures and is suitable as suspension buffer for biological samples.
NZYTech’s Tris buffer is supplied as exactly pre-weighed powder in pouches at pH 8.0, one pouch giving 1000 mL of 1 M Tris-HCl buffer at 25°C.
Features:
– Formulated from analytical grade reagents
– Wide buffering capacity range: pH 7.0 to 9.2
– Dissolve and go for greater convenience consistency from lot to lot
Applications:
– Component in TAE and TBE buffers
– Cell and tissue culture procedures
– Biochemistry and molecular biology
Directions for use: Empty one pouch of the Tris buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 mL of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 mL, stir until full dissolution and the buffer is ready to use.
Specifications:
– Chemicals: Analytical grade
– Format: Exactly pre-weighed powder mix
– Concentration: 1 M Tris-HCl
– Volume: 1000 mL
– pH: 8.0 ± 0.05 at 25 °C
Storage: Store the pouches in a dry place at room temperature. Tris buffer solutions can be stored at room temperature or at 4 °C for two weeks.
