X-GAL (MB025)


Description: X-Gal is the abbreviation for 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside; less commonly the abbreviation BCIG is also used. X-Gal is enzymatically hydrolysed by β-galactosidase to yield galactose and 5-bromo-4-chloro-3-hydroxyindole. The soluble, colourless, indole hydrolysis product is subsequently transformed into an insoluble blue compound, 5,5’-dibromo-4,4’-dichloro indigo, via a dimerisation and oxidation sequence. X-Gal is frequently used in molecular biology applications to indicate the activity of β-galactosidase, an enzyme encoded by the lacZ gene, a classical reporter gene. In gene cloning, a X-Gal blue/white colony screening assay can be used to differentiate recombinant colonies which stain white from non-recombinant ones which stain blue.

Chemical formula: C14H15BrClNO6

MW: 408.63

CAS number: 7240-90-6

Soluble in: DMSO and DMF

Storage: -20 ºC

Certificate of Analysis ZN111
Certificate of Analysis YO081

Cloning, Expression and Characterization of UDP-Glucose Dehydrogenases
Couto M, Rodrigues J, Rodrigues L
Life, 2021

A Combinatorial Approach to Optimize the Production of Curcuminoids From Tyrosine in Escherichia coli
Rodrigues JL, Gomes D, Rodrigues LR
Front Bioeng Biotechnol, 2020

Selection of Novel Peptides Homing the 4T1 CELL Line: Exploring Alternative Targets for Triple Negative Breast Cancer
Silva VL, Ferreira D, Nobrega FL, Martins IM, Kluskens LD, Rodrigues LR
PLoS One, 2016

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