Description: Recommended medium for the test of Escherichia coli in molecular genetics studies. LB Agar Lennox is nutritionally rich medium developed by Lennox for the growth and maintenance of pure culture of recombinant strains of E. coli. Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent. If desired, antibiotics can also be added.
Directions for use: Suspend 35 grams of medium in 1 L distilled water. Mix well and dissolve by heating and stirring. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC, mix well and dispense into plates.
– Tryptone: 10 g/L
– Sodium Chloride: 5 g/L
– Yeast Extract: 5 g/L
– Bacteriological Agar: 15 g/L
(Final pH 7.0± 0.2 at 25 ºC)
Soluble in: water
Storage: Store at 2-25 ºC. Once opened keep powdered medium closed to avoid hydration. Protect form light and moist