NZY5α (MB004)

Description: NZY5α Competent Cells display similar properties to DH5α, which are suitable for high efficiency transformation in a wide variety of applications. The φ80dlacZ∆M15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors and, therefore, can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. NZY5α can also serve as a host for the M13mp cloning vectors. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α™ Cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Features:
– Transformation efficiency: ≥ 10⁹ cfu/μg of pNZY28
– Blue/White screening capability

Applications:
– Routine cloning
– High quality plasmid preparation
– Hosting M13mp cloning vectors

Specifications:
– Genotype: fhuA2∆(argF-lacZ)U169 phoA glnV44 Φ80 ∆(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
– Storage conditions: Store at -80 ºC
– Shipping conditions: Shipped at dry ice

Components:
– NZY5α Competent Cells (10 or 20 × 200 µL)
– Competent Cells Control Plasmid (10 µL at 0.1 ng/µL)