NZY5α (MB004)

Refrigerated package


Description: NZY5α Competent Cells display similar properties to DH5α, which are suitable for high efficiency transformation in a wide variety of applications. The φ80dlacZ∆M15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors and, therefore, can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. NZY5α can also serve as a host for the M13mp cloning vectors. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α™ Cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

– Transformation efficiency: ≥ 109 cfu/μg of pNZY28
– Blue/White screening capability

– Routine cloning
– High quality plasmid preparation
– Hosting M13mp cloning vectors

– Genotype: fhuA2∆(argF-lacZ)U169 phoA glnV44 Φ80 ∆(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
– Storage conditions: Store at -80 ºC
– Shipping conditions: Shipped at dry ice

– NZY5α Competent Cells (10 or 20 × 200 µL)
– Competent Cells Control Plasmid (10 µL at 0.1 ng/µL)

Product Brochure EN
CoA Certificate of Analysis NP012
CoA Certificate of Analysis NP011
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN022
Certificate of Analysis ZN021
Certificate of Analysis ZN012
Certificate of Analysis ZN011
Certificate of Analysis YO122
Certificate of Analysis YO121
Certificate of Analysis YO111
Certificate of Analysis YO102
Certificate of Analysis YO101
Certificate of Analysis YO093
Certificate of Analysis YO092
Certificate of Analysis YO091
Certificate of Analysis YO073
Certificate of Analysis YO072
Certificate of Analysis YO071
Certificate of Analysis YO062
Certificate of Analysis YO061
Certificate of Analysis YO042
Certificate of Analysis YO041
Certificate of Analysis YL101
Certificate of Analysis NM104
Certificate of Analysis NM103
Certificate of Analysis NM102
Certificate of Analysis NM101
Certificate of Analysis NM074
Certificate of Analysis NM073
Certificate of Analysis NM072
Certificate of Analysis NM071

Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae
Rainha J, Rodrigues J, aria C, Rodrigues L
Biotechnology Journal, 2021

Cloning, Expression and Characterization of UDP-Glucose Dehydrogenases
Couto M, Rodrigues J, Rodrigues L
Life, 2021

In vitro selection of DNA aptamers against human osteosarcoma
Tsogtbaatar K, Sousa DA, Ferreira D, Tevlek A, Aydin HM, Celik E, Rodrigues L
Investigation New Drugs, 2021

A Combinatorial Approach to Optimize the Production of Curcuminoids From Tyrosine in Escherichia coli
Rodrigues JL, Gomes D, Rodrigues LR
Front Bioeng Biotechnol, 2020

Tet3 regulates cellular identity and DNA methylation in neural progenitor cells
Santiago M, Antunes C, Guedes M, Iacovino M, Kyba M, Reik W, Sousa N, Pinto L, Branco M, Marques CJ
Cellular and Molecular Life Sciences, 2019

Physiological characterization of a pyrimidine auxotroph exposes link between uracil phosphoribosyltransferase regulation and riboflavin production in Ashbya gossypii
Silva R, Aguiar TQ, Oliveira C, Domingues L
N Biotechnol, 2019

Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways
Cunha JT, Soares PO, Romani A, Thevelein JM, Domingues L
Biotechnol Biofuels, 2019

The Crystal Structure of the R280K Mutant of Human p53 Explains the Loss of DNA Binding.
Gomes AS, Trovão F, Andrade Pinheiro B, Freire F, Gomes S, Oliveira C, Domingues L, Romão MJ, Saraiva L, Carvalho AL
Int J Mol Sci, 2018

RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: Adulterant sheep breeds in Serra da Estrela cheese production
Cunha JT, Ribeiro TI, Rocha JB, Nunes J, Teixeira JA, Domingues L
Food Chemistry, 2016

Production of curcuminoids from tyrosine by a metabolically engineered Escherichia coli using caffeic acid as an intermediate
Rodrigues JL, Araújo RG, Prather KL, Kluskens LD, Rodrigues LR
Biotechnol J, 2015

A tailor-made “tag-receptor” affinity pair for the purification of fusion proteins
Pina AS, Guilherme M, Pereira AS, Fernandes CS, Branco RJ, El Khoury G, Lowe CR, Roque AC
Chembiochem, 2014


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