Description: BL21(DE3) Competent Cells are chemically competent Escherichia coli cells used for protein expression of T7 RNA Polymerase-based systems. These cells are resistant to the lytic bacteriophages T1 and T5. The BL21 (DE3) strain is a derivative of E. coli B. It is deficient in both lon and ompT proteases resulting in superior isolation of intact recombinant proteins. The host is a lysogen of DE3 and, therefore, carries a chromosomal copy of the T7 RNA polymerase gene that is controlled by the lacUV5 promoter. The strain utilizes the T7 RNA promoter to control protein expression. IPTG is used to induce expression of the T7 RNA polymerase.
Features:
– Transformation efficiency: ≥ 106 cfu/μg of pNZY28
– High-level of protein expression
– Deficient in proteases Ion and OmpT
Applications:
– Protein expression using the T7 expression system
Specifications:
– Genotype: F¯ ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 *lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
– Storage conditions: Store at -80 ºC
– Shipping conditions: Shipped at dry ice
Components:
– B21(DE3) Competent Cells (10 or 20 × 200 µL)
– Competent Cells Control Plasmid (10 µL at 0.1 ng/µL)
