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NZYMutagenesis kit
NZYMutagenesis kit provides a simple and highly efficient method to generate point mutations and delete or insert single (or multiple) nucleotides in any type of plasmid DNA using PCR.
Availability
Ready to Ship
Dry Ice
NZYMutagenesis kit is designed to make point mutations and delete or insert single or multiple nucleotides in a DNA sequence. The system requires the provision of two synthetic oligonucleotide primers containing the desired mutation. Incorporation of the oligonucleotide primers with NZYProof DNA polymerase generates a mutated plasmid containing staggered nicks, which resists Dpn Idigestion (as the synthetic DNA is not methylated). The resulting mutated plasmid is recovered through transformation of NZYStar competent cells. For a schematic presentation of the mutagenesis protocol, see Figure 1. DNA isolated from dam- Escherichia coli strains, including JM101 and SCS110, is not a suitable template forthe mutagenesis reaction.
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | Competent cells: -85 °C to -65 °C / Other components: -30 °C to -15 °C |
| Application | Site-directed mutagenesis |
| Features | Mutagenesis of plasmids <15 kb |
| Product Category | DNA Cloning & Mutagenesis |
– Reaction Buffer (10x)
– dNTP mix
– NZYProof DNA polymerase (2.5 U/µL)
– Dpn I (10 U/µL)
– Control plasmid
– Control primer mix
– NZYStar Competent Cells (*)
– Competent Cells Control Plasmid (0.1 ng/µL) (*)
(*) only provided in MB01202 kit
– dNTP mix
– NZYProof DNA polymerase (2.5 U/µL)
– Dpn I (10 U/µL)
– Control plasmid
– Control primer mix
– NZYStar Competent Cells (*)
– Competent Cells Control Plasmid (0.1 ng/µL) (*)
(*) only provided in MB01202 kit
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | Competent cells: -85 °C to -65 °C / Other components: -30 °C to -15 °C |
| Application | Site-directed mutagenesis |
| Features | Mutagenesis of plasmids <15 kb |
| Product Category | DNA Cloning & Mutagenesis |
The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
Raquel M. Bernardino
PNAS, 2021
A protein trisulfide couples dissimilatory sulfate reduction to energy conservation
Santos AA, Venceslau SS, Grein F, Leavitt WD, Dahl C, Johnston DT, Pereira IA
Science, 2015
Interaction studies between periplasmic cytochromes provide insights into extracellular electron transfer pathways of Geobacter sulfurreducens
Fernandes AP, Nunes TC, Paquete CM, Salgueiro CA
Biochem J, 2017
Exploring the molecular mechanisms of electron shuttling across the microbe/metal space
Paquete CM, Fonseca BM, Cruz DR, Pereira TM, Pacheco I, Soares CM, Louro RO
Front Microbiol, 2014
Ser119 phosphorylation modulates the activity and conformation of PRRXL1, a homeodomain transcription factor
Soares-Dos-Reis R, Pessoa A S, Matos M R, Falcão M, Mendes VM, Manadas B, Monteiro F A, Lima D, Reguenga C
Biochemical Journal, 2014
Dual role of Tlx3 as modulator of Prrxl1 transcription and phosphorylation
Regadas I, Soares-Dos-Reis R, Falcão M, Matos MR, Monteiro FA, Lima D, Reguenga C
Biochimica et Biophysica Acta, 2014"
The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase
Raquel M. Bernardino
PNAS, 2021
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | Competent cells: -85 °C to -65 °C / Other components: -30 °C to -15 °C |
| Application | Site-directed mutagenesis |
| Features | Mutagenesis of plasmids <15 kb |
| Product Category | DNA Cloning & Mutagenesis |
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | Competent cells: -85 °C to -65 °C / Other components: -30 °C to -15 °C |
| Application | Site-directed mutagenesis |
| Features | Mutagenesis of plasmids <15 kb |
| Product Category | DNA Cloning & Mutagenesis |
CoA
Certificate of Analysis