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NZY-blunt PCR cloning kit
NZY-blunt PCR cloning kit was designed to allow the direct cloning of PCR products with blunt ends, which result from PCR amplifications using proofreading DNA polymerases, such as NZYProof DNA polymerase, into pNZY28.
Availability
Ready to Ship
Dry Ice
NZY-blunt PCR cloning kit was designed to allow the direct cloning of PCR products with blunt ends, which result from PCR amplifications using proofreading DNA polymerases, into pNZY28. The pNZY28 vector provided was linearized at the EcoR V and dephosphorilated.
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | Competent cells: -85 °C to -65 °C –Other components: -30 °C to -15 °C |
| Application | Blunt-end Cloning |
| Features | Blue/White screening |
| Product Category | DNA Cloning & Mutagenesis |
| Protocol time | Approx. 60 min |
– Phos Buffer (10x)
– Phos Enzyme Mix
– Positive control insert (120 ng/µL)
– Linearized pNZY28 vector (20 ng/µL)
– T4 DNA Ligase (2 U/µL)
– NZYStar Competent Cells (*)
– Competent Cells Control Plasmid (0.1 ng/µL) (*)
(*) only provided in MB12101 kit
– Phos Enzyme Mix
– Positive control insert (120 ng/µL)
– Linearized pNZY28 vector (20 ng/µL)
– T4 DNA Ligase (2 U/µL)
– NZYStar Competent Cells (*)
– Competent Cells Control Plasmid (0.1 ng/µL) (*)
(*) only provided in MB12101 kit
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | Competent cells: -85 °C to -65 °C –Other components: -30 °C to -15 °C |
| Application | Blunt-end Cloning |
| Features | Blue/White screening |
| Product Category | DNA Cloning & Mutagenesis |
| Protocol time | Approx. 60 min |
Identification of a novel human LAP1 isoform that is regulated by protein phosphorylation
Santos M, Domingues SC, Costa P, Muller T, Galozzi S, Marcus K, da Cruz e Silva EF, da Cruz e Silva OA, Rebelo S
PLos One, 2014
Santos M, Domingues SC, Costa P, Muller T, Galozzi S, Marcus K, da Cruz e Silva EF, da Cruz e Silva OA, Rebelo S
PLos One, 2014
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | Competent cells: -85 °C to -65 °C –Other components: -30 °C to -15 °C |
| Application | Blunt-end Cloning |
| Features | Blue/White screening |
| Product Category | DNA Cloning & Mutagenesis |
| Protocol time | Approx. 60 min |
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | Competent cells: -85 °C to -65 °C –Other components: -30 °C to -15 °C |
| Application | Blunt-end Cloning |
| Features | Blue/White screening |
| Product Category | DNA Cloning & Mutagenesis |
| Protocol time | Approx. 60 min |
CoA
Certificate of Analysis
