Klenow Fragment of DNA Polymerase I (MB00901)

Refrigerated package

69,00

300 U

69,00

Description: Klenow Fragment of DNA Polymerase I is a truncated fragment of E. coli DNA polymerase I that lacks the 5′ → 3′ exonuclease activity while retaining the 3′ → 5′ exonuclease activity (proofreading).

Features:
– Free from detectable RNase and DNase activities
– Flexible for a range of molecular applications
– Can incorporate modified nucleotides (e.g. biotin, digoxigenin, fluorescently, labeled nucleotides)
– Supplied with an optimized reaction buffer for efficient activity

Applications:
– 3´-end labelling of DNA fragments for sequence analysis
– Conversion of 5´-overhangs to create blunt-ends for cloning
– Removal of 3´-overhangs
– Second-strand synthesis of cDNA
– Random primer labelling of DNA fragments

Specifications:

Enzyme concentration:5 U/μL
Source:Recombinant E. coli strain
Optimal reaction temperature:37 °C
Heat inactivation:75 °C for 10 min
Storage conditions:Store at -20 °C
Shipping conditions:Shipped at dry ice

Components:
– Klenow Fragment of DNA Polymerase I (300 U)
– Reaction buffer (10x)

MSDS EN
MSDS PT
Product Brochure EN

You may also like…

NZY First-Strand cDNA Synthesis Kit

169,00749,00
Clear

Phospho-β-Glucosidase 1B

129,00299,00
Clear

β-Glucosidase 1A

129,00299,00
Clear