Description: Alkaline Phosphatase is suitable for removal of terminal monoesterified phosphates from deoxyribo-oligonucleotides. Used for the removal of single phosphate groups from 5´-ends of linear vectors to prevent re-circularization during cloning or to dephosphorylate DNA prior to kinase labelling protocols.
– Free from detectable nonspecific nuclease, endonuclease, RNase and DNase activities
– Very stable enzyme
– Supplied with an optimized reaction buffer containing Zn2+ for efficient activity
– Dephosphorylation of DNA
– Treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis
|Enzyme concentration:||0.5 U/μL|
|Source:||Recombinant E. coli strain|
|Optimal reaction temperature:||37 °C|
Temporarily inactivated after incubation at 100 °C in the presence of a chelating reagent (the enzyme recovers activity when returned to room temperature). Phenol extraction or DNA purification using NZYTech ́s NZYGelpure is, therefore, recommended.
|Storage conditions:||Store at -20 °C|
|Shipping conditions:||Shipped at dry ice|
– Alkaline Phosphatase (200 U)
– Reaction buffer (10x)