Description: T4 DNA Ligase is an ultrapure recombinant enzyme purified from Escherichia coli supplied with an optimized 10× Reaction Buffer, which includes ATP. T4 DNA Ligase catalyses the formation of a phosphodiester bond between juxtaposed 5′-phosphoryl and 3′-hydroxyl termini in duplex DNA. It repairs single-strand nicks in duplex DNA and will join both blunt and cohesive-end restriction fragments of duplex DNA or RNA. The enzyme requires ATP as cofactor.
Features:
– Free from detectable nonspecific nuclease, endonuclease, RNase and DNase activities
– Supplied with an optimized reaction buffer for efficient ligation reactions
Applications:
– Cloning of both blunt- and sticky (cohesive)-end restriction fragments
– Cloning of PCR products
– Joining linkers and adapters to DNA
– Nick repair
– Self-circularization of linear DNA
Specifications:
| Enzyme concentration: | 5 U/μL |
| Source: | Recombinant E. coli strain |
| Optimal reaction temperature: | 16-20 °C |
| Heat inactivation: | 65 °C for 10 min |
| Storage conditions: | Store at -20 °C |
| Shipping conditions: | Shipped at dry ice |
Components:
– T4 DNA Ligase (5 U/μL)
– Reaction buffer (4x)
