Description: Speedy Ligase is an improved DNA Ligase developed to carry out fast (less than 15 minutes) and efficient ligation of sticky or blunt-end DNA at room temperature. The enzyme catalyses the formation of a phosphodiester bond between juxtaposed 5′-phosphoryl and 3′-hydroxyl termini in duplex DNA. Rapid ligation is based on the combination of Speedy Ligase with a 4× Speedy ligation buffer.
Features:
– Fast (5 min for cohesive-ends or 15 min for blunt-ends at room temperature)
– Free from detectable nonspecific nuclease, endonuclease, RNase and DNase activities
– Supplied with an optimized reaction buffer for efficient ligation reactions
Applications:
– Cloning of both blunt- and sticky (cohesive)-end restriction fragments
– Cloning of PCR products
– Joining linkers and adapters to DNA
– Nick repair
– Self-circularization of linear DNA
Specifications:
| Enzyme concentration: | 10 U/μL |
| Optimal reaction temperature: | room temprature (20-25 °C) |
| Heat inactivation: | 65 °C for 10 min |
| Storage conditions: | Store at -20 °C |
| Shipping conditions: | Shipped at dry ice |
Components:
– Speedy Ligase (10 U/μL)
– Reaction buffer (4x)
