Speedy Ligase (MB13001)

Refrigerated package

81,00

50 ligations

81,00

Description: Speedy Ligase is an improved DNA Ligase developed to carry out fast (less than 15 minutes) and efficient ligation of sticky or blunt-end DNA at room temperature. The enzyme catalyses the formation of a phosphodiester bond between juxtaposed 5′-phosphoryl and 3′-hydroxyl termini in duplex DNA. Rapid ligation is based on the combination of Speedy Ligase with a 4× Speedy ligation buffer.

Features:
– Fast (5 min for cohesive-ends or 15 min for blunt-ends at room temperature)
– Free from detectable nonspecific nuclease, endonuclease, RNase and DNase activities
– Supplied with an optimized reaction buffer for efficient ligation reactions

Applications:
– Cloning of both blunt- and sticky (cohesive)-end restriction fragments
– Cloning of PCR products
– Joining linkers and adapters to DNA
– Nick repair
– Self-circularization of linear DNA

Specifications:

Enzyme concentration: 10 U/μL
Optimal reaction temperature: room temprature (20-25 °C)
Heat inactivation: 65 °C for 10 min
Storage conditions: Store at -20 °C
Shipping conditions: Shipped at dry ice

Components:
– Speedy Ligase (10 U/μL)
– Reaction buffer (4x)

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN112
Certificate of Analysis ZN031
Certificate of Analysis YO061

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