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SKU
MB42401

DNA Ligase E. coli

Storage Conditions:
-85 °C to -15 °C
DNA Ligase (E. coli) is a typical DNA ligase that catalyses the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands in duplex DNA.
Availability
Ready to Ship
Dry Ice
Unit Size
200 U
€65.00
DNA Ligase (E. coli) is a typical DNA ligase that catalyses the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands in duplex DNA. Thus, the enzyme is particularly effective to repair nicks in dsDNA although it can be used to ligate two DNA fragments with compatible cohesive ends. DNA Ligase (E. coli) is not significantly active on blunt-ended substrates. DNA Ligase (E. coli) uses NAD as a cofactor and can be heat-inactivated. The enzyme is active at a range of temperatures (4 °C - 37 °C).
Applications:
– Gene cloning
– Second-strand cDNA synthesis
– Phosphodiester bond ligations
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity Sticky-end ligation
Product Category Ligases
Reaction Temperature 16 °C
Heat Inactivation 65 °C
Concentration 10 U/μL
– DNA Ligase (E. coli) (10 U/μL)
– Reaction buffer (10x)
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity Sticky-end ligation
Product Category Ligases
Reaction Temperature 16 °C
Heat Inactivation 65 °C
Concentration 10 U/μL
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity Sticky-end ligation
Product Category Ligases
Reaction Temperature 16 °C
Heat Inactivation 65 °C
Concentration 10 U/μL

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