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SKU
MB43101

Endonuclease IV (Tth)

Storage Conditions:
-85 °C to -15 °C
Endonuclease IV (Tth) is a Class II thermostable Apurinic/aPyrimidinic (AP) DNA endonuclease from Thermus thermophilus. This an enzyme is involved in the DNA Base Excision Repair (BER) pathway.
Availability
Ready to Ship
Dry Ice
Unit Size
500 U
€76.00
Endonuclease IV (Tth) is a Class II thermostable Apurinic/aPyrimidinic (AP) DNA endonuclease from Thermus thermophilus. This an enzyme is involved in the DNA Base Excision Repair (BER) pathway, that catalyses the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis, leaving a 1 nucleotide gap with 3′-hydroxyl and 5′ deoxyribose phosphate (dRP) termini. AP sites are locations in DNA that neither contain a purine nor a pyrimidine base, which occur either spontaneously or due to DNA damage. Endonuclease IV (Tth) displays activity in both ssDNA and dsDNA. The enzyme also displays 3’-diesterase activity.
Applications:
– Studies of DNA damage
– Single cell electrophoresis (comet assay)
– Alkaline unwinding
– SNP analysis
– Genotyping
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity 3′ → 5′ ssDNA nuclease, Nicking Endonuclease
Product Category Nucleases
Reaction Temperature 65°C
Heat Inactivation No
Concentration 10 U/μL
– Endonuclease IV (Tth) (10 U/μL)
– Reaction Buffer (10x)
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity 3′ → 5′ ssDNA nuclease, Nicking Endonuclease
Product Category Nucleases
Reaction Temperature 65°C
Heat Inactivation No
Concentration 10 U/μL
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity 3′ → 5′ ssDNA nuclease, Nicking Endonuclease
Product Category Nucleases
Reaction Temperature 65°C
Heat Inactivation No
Concentration 10 U/μL

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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