Description: Exonuclease I (E. coli) is a typical DNA specific exonuclease that catalyses the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction. The enzyme may be used to remove single-stranded primers in PCR reactions prior to Sanger DNA sequencing or SNP analysis nested PCR reactions. Exonuclease I (E. coli) may be used to generally remove linear single-stranded DNA, leaving behind double-stranded DNA in the sample.
Features:
– DNA specific exonuclease
– 3′ → 5′ single-stranded nuclease activity
Applications:
– Removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction
– Removal of single-stranded primers in PCR mixture prior to Sanger DNA sequencing
– Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample
Specifications:
– Enzyme concentration: 20 U/μL
– Source: Recombinant E. coli strain
– Optimal reaction temperature: 37 °C
– Heat inactivation: 80 °C for 15 min
– Storage conditions: Store at -20 °C
– Shipping conditions: Shipped at dry ice
Components:
– Exonuclease I (E.coli) (20 U/μL)
– Reaction Buffer (10x)
