Notification Bar Icon

Unlock 5% OFF: Shop online now and save on every purchase! Limited time offer.

Unlock 5% OFF: Shop online now and save on every purchase! Limited time offer.Learn more

Learn more
SKU
MB43001

Exonuclease III (E. coli)

Storage Conditions:
-85 °C to -15 °C
Exonuclease III (E. coli) is a typical double-stranded DNA specific nuclease that cleaves DNA from the 3′ termini of double-stranded DNA with 5′ overhangs or blunt ends and 3′ overhangs containing less than four bases.
Availability
Ready to Ship
Dry Ice
Unit Size
5000 U
€65.00
Exonuclease III (E. coli) is a typical double-stranded DNA specific nuclease that cleaves DNA from the 3′ termini of double-stranded DNA with 5′ overhangs or blunt ends and 3′ overhangs containing less than four bases. The enzyme also initiates cleavage at nicked sites in double-stranded DNA although this is not the preferred substrate. The enzyme is not active on single-stranded DNA. Thus, exonuclease III (E. coli) is an enzyme that catalyses the removal of nucleotides from linear or nicked double-stranded DNA in the 3′ to 5′ direction. Exonuclease III (E. coli) has also been reported to display residual RNase H, 3´-phosphatase and AP-endonuclease activities.
Applications:
– Removal of nucleotides from linear or nicked double-stranded DNA
– Generation of single-stranded DNA for dideoxy sequencing
– Site-directed mutagenesis
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity 3′ → 5′ dsDNA nuclease
Product Category Nucleases
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Concentration 100 U/μL
– Exonuclease III (E. coli) (100 U/μL)
– Reaction Buffer (10x)
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity 3′ → 5′ dsDNA nuclease
Product Category Nucleases
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Concentration 100 U/μL
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity 3′ → 5′ dsDNA nuclease
Product Category Nucleases
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Concentration 100 U/μL

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

Related Products