Exonuclease VII ( E. coli ) (MB43201)

Refrigerated package

69,00

200 U

69,00

Description: Exonuclease VII (E. coli) is a typical DNA specific exonuclease that cleaves linear single-stranded DNA in both the 3′ to 5′ and 5′ to 3′ directions. The enzyme maybe used for the removal of primers with or without 3′ or 5′ terminal phosphorothioate bonds or to map positions of introns in genomic DNA. Generally, Exonuclease VII (E. coli) is used to remove single-stranded DNA, leaving behind the double-stranded DNA in a sample, in particular in PCR reactions. This enzyme is not active on linear or circular dsDNA.

Features:
– DNA specific exonuclease
– Catalyses the cleavage of linear single-stranded DNA in both the 3′ to 5′ and 5′ to 3′ directions
– No active on linear or circular dsDNA

Applications:
– Removal of single-stranded oligonucleotide primers after PCR
– Removal of single-stranded DNA from samples with dsDNA
– Removal of oligonucleotide primers with or without 3′ or 5′ terminal phosphorothioate
– Mapping positions of introns in genomic DNA
– Removal of single-stranded overhangs generated by restriction endonucleases

Specifications:

– Enzyme concentration: 10 U/μL
– Source: Recombinant E. coli strain
– Optimal reaction temperature: 37 °C
– Heat inactivation: 95 °C for 10 min
– Storage conditions: Store at -20 °C
– Shipping conditions: Shipped at dry ice

Components:
– Exonuclease VII (E. coli) (10 U/μL)
– Reaction Buffer (5x)

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN051
Certificate of Analysis NZ051

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